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目的观察大鼠大脑白质、皮质内毛细血管总长度、总体积、总表面积和平均直径。方法选5只7月龄Long-Evans大鼠。运用免疫组织化学技术及相应的体视学方法 ,计算白质、皮质内毛细血管的长度密度、体积密度﹑表面积密度和毛细血管的总长度、总体积﹑总表面积、平均直径。结果大鼠白质由于组织处理引起的白质体积皱缩率为0.51±0.09。白质毛细血管的总长度、总体积、总表面积、平均直径依次为(30.9±6.2)m、(0.8±0.09)mm3、(5.3±0.74)cm2、(5.5±1.1)μm。皮质由于组织处理引起的体积皱缩率为0.57±0.08。皮质毛细血管的总长度、总体积、总表面积、平均直径的近似值依次为(364.8±68.0)m、(7.0±1.6)mm3、(51.6±11.7)cm2、(4.5±0.40)μm。结论本研究方法为以后同类型的研究提供了较为理想的方法学借鉴。本研究结果为将来的研究提供了大鼠大脑白质和皮质毛细血管参数的基本数值。
Objective To observe the total length of capillary, total volume, total surface area and average diameter of white matter and cortex in rat brain. Methods Five 7-month-old Long-Evans rats were selected. Immunohistochemistry and corresponding stereological methods were used to calculate the length, capillary density, surface area density, total capillary length, total volume, total surface area, and average diameter of capillaries in white matter and cortex. Results The white matter volume shrinkage rate of white matter in rats was 0.51 ± 0.09. The total length, total volume, total surface area and average diameter of white blood capillaries were (30.9 ± 6.2) m, (0.8 ± 0.09) mm3, (5.3 ± 0.74) cm2 and (5.5 ± 1.1) μm, respectively. Cortical volume shrinkage due to tissue processing was 0.57 ± 0.08. The approximate values of total length, total volume, total surface area and average diameter of cortical capillaries were (364.8 ± 68.0) m, (7.0 ± 1.6) mm3, (51.6 ± 11.7) cm2 and (4.5 ± 0.40) μm, respectively. Conclusion The research method provided an ideal methodological reference for the same type of research in the future. The results of this study provide basic values for white matter and cortical capillary parameters in rat brain for future studies.