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本文利用ＴＰＯ基因注射治疗接受环磷酰胺化疗后小鼠的血小板及白细胞减少症。将克隆于ｐｃＤＮＡ３的ＴＰＯ质粒按不同剂量注射到试验组２７只小鼠后肢内侧肌肉内，其中３只小鼠注射空质粒。三日后其中接受ＴＰＯ基因注射的１２只小鼠每只腹腔内注射环磷酰胺２ｍｇ（１００ｍｇ／ｋｇ体重），其余１２只不化疗的小鼠作为基因对照组；另设化疗对照组动物１５只，按相同剂量注射环磷酰胺。每周尾静脉采血，检测白细胞和血小板水平。结果基因＋化疗组的两项血液指标在化疗后两周内明显优于单纯化疗组，无毛发脱落；化疗组和注射空质粒组动物绝大多数发生脱毛现象，并有７只动物死亡。两周后进行第二次环磷酰胺攻击，结果化疗组的死亡数达８只，存活动物体况很差，基因＋化疗组动物尚无任何不良表现。此时两组的血液指标已无明显差异，提示在基因注射后ＴＰＯ的有效表达期在２－３周。本研究发现ＴＰＯ基因注射后，不但动物血小板和白细胞水平提高，而且其它化疗后的不良症状也得到改善，提示ＴＰＯ有着更为广泛的作用
In this article, TPO injection was used to treat platelets and leukopenia in mice receiving chemotherapy with cyclophosphamide. The TPO plasmid cloned in pcDNA3 was injected at different doses into the inner muscles of 27 mice of the experimental group, and 3 mice were injected with empty plasmids. Three days later, 12 mice that received TPO injection were intraperitoneally injected with 2 mg cyclophosphamide (100 mg/kg body weight) intraperitoneally, and the remaining 12 non-chemotherapy mice were used as a gene control group; 15 animals were also given a chemotherapy control group. Cyclophosphamide was injected at the same dose. Blood was collected from the tail vein weekly to detect white blood cell and platelet levels. Results The two blood parameters in the gene + chemotherapy group were significantly better than the chemotherapy alone group within two weeks after chemotherapy, and no hair loss occurred. The majority of animals in the chemotherapy group and the injected plasmid group had epilation, and 7 animals died. After a second cyclophosphamide challenge two weeks later, the number of deaths in the chemotherapy group was 8 and the condition of the active animals was poor. The animals in the gene + chemotherapy group did not have any adverse symptoms. There was no significant difference in blood parameters between the two groups at this time, suggesting that the effective expression period of TPO after gene injection was 2-3 weeks. In this study, it was found that after the TPO gene was injected, not only the platelet and leukocyte levels of the animals were elevated, but also other adverse symptoms after chemotherapy were also improved, suggesting that TPO has a more extensive role.