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目的:探讨RhoA、ROCK1和ROCK2蛋白在乳腺浸润性导管癌组织中的表达。方法:用免疫组织化学检测RhoA、ROCK1、ROCK2、雌激素ER在35例乳腺浸润性导管癌组织中的表达。结果:与病理分级Ⅰ～Ⅱ级比较,病理分级Ⅲ级RhoA蛋白表达减少,ROCK1蛋白表达明显增加,ROCK2蛋白表达明显减少。与淋巴结未转移组比较,有淋巴结转移组RhoA蛋白和ROCK1蛋白表达增加,ROCK2蛋白表达明显增加。与ER蛋白阴性组相比,ER蛋白阳性组RhoA蛋白表达增加,ROCK1蛋白表达减少,ROCK2蛋白表达增加。RhoA蛋白高表达时,ROCK1蛋白低表达,但无相关性(r=-0.259,P>0.05);RhoA蛋白高表达时,ROCK2蛋白高表达,呈显著正相关(r=0.673,P<0.05);ROCK1蛋白高表达时,ROCK2蛋白低表达,但无相关性(r=-0.087,P>0.05)。结论:ROCK2蛋白高表达可能促进乳腺癌淋巴结转移,RhoA与ROCK信号途径有可能成为乳腺癌治疗靶点。
Objective: To investigate the expression of RhoA, ROCK1 and ROCK2 in breast invasive ductal carcinoma. Methods: The expressions of RhoA, ROCK1, ROCK2 and estrogen ER in 35 cases of breast invasive ductal carcinoma were detected by immunohistochemistry. Results: Compared with the pathological grade Ⅰ ~ Ⅱ, the expression of RhoA protein in pathological grade Ⅲ was decreased, the expression of ROCK1 protein and ROCK2 protein were significantly decreased. Compared with lymph node metastasis group, the expression of RhoA protein and ROCK1 protein increased and ROCK2 protein expression increased significantly in lymph node metastasis group. Compared with the ER-negative group, the expression of RhoA protein, ROCK1 protein and ROCK2 protein increased in ER-positive group. There was a significant positive correlation between the expression of ROCK2 protein and ROCK protein (r = -0.259, P <0.05) when RhoA protein was overexpressed (r = -0.259, P> 0.05) There was no correlation between ROCK1 protein and ROCK1 protein expression (r = -0.087, P> 0.05). Conclusion: The high expression of ROCK2 may promote the lymph node metastasis of breast cancer. The RhoA and ROCK signal pathways may be the target of breast cancer therapy.