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本实验比较654-2与异搏定对大鼠缺氧肝脏溶酶体酶释放的抑制作用,以探讨654-2的细胞保护作用与阻断细胞膜钙离子慢通道的关系。大鼠随机分为3组:(1)对照组:用krebs-Hen seleit液(氧分压60-90mmHg)经门靜脉灌流离体大鼠肝脏2小时,分别测定灌流开始,60、90、120分钟肝脏流出液中组织蛋白酶D的活性,灌流结束后称取1 g肝组织制备匀浆,用差速离心法分离肝溶酶体,分别测定游离组织蛋白酶D(溶酶体外酶活性)和结
This experiment compared the inhibitory effect of 654-2 and verapamil on rat liver lysosomal enzyme release in order to explore the relationship between the protective effect of 654-2 and block the slow pathway of calcium ion in the cell membrane. Rats were randomly divided into three groups: (1) control group: isolated rat liver was perfused with Krebs-Hen seleit solution (oxygen partial pressure of 60-90 mmHg) for 2 hours through portal vein to measure the perfusion, 60, 90, 120 Min liver cathepsin D activity in the effusion, after the end of perfusion, weighed 1 g of liver tissue preparation of the homogenate by differential centrifugation hepatic lysosomes were measured free cathepsin D (lysosomal enzyme activity) and knot