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目的探讨脓毒血症大鼠模型心脏5-羟色胺改变与心肌动作电位变化之间的关系。方法利用随机数字表将大鼠分为脓毒血症组和正常对照组,每组各10只,进行脓毒血症模型制作,18 h后进行心脏病理切片、电生理实验、5-羟色胺免疫组化实验并定量分析。结果脓毒血症组心脏线粒体发生改变,其心率[(353.60±11.27)次/min]、心房动作电位时程[(112.16±8.97)ms]均较正常组[心率(306.10±11.21)次/min、心房动作电位时程(86.31±7.49)ms]延长(P<0.01),心肌细胞阳性面积[(19 193.38±588.13)μm2]和阳性细胞数[(18.50±3.37)个/视野]较正常组[(7 755.01±586.16)μm2、(13.30±1.49)个/视野]均明显增加(P<0.05,P<0.01)。结论 5-羟色胺是一种炎性因子,导致心房动作电位时程延长、心率增快,加重心肌损伤,也是导致脓毒血症心功能障碍的众多因素之一。
Objective To investigate the relationship between changes of myocardial 5-hydroxytryptamine and changes of myocardial action potential in septic rat model. Methods The rats were divided into sepsis group and normal control group by random number table. Each group consisted of 10 rabbits. The models were made by sepsis model. The pathological sections were made after 18 hours. Electrophysiology, serotonin immunization Tissue experiments and quantitative analysis. Results Cardiac mitochondria were changed in sepsis group with heart rate [(353.60 ± 11.27) / min] and duration of atrial action potential [(112.16 ± 8.97) ms] compared with those in normal group [heart rate (306.10 ± 11.21) min, the duration of atrial action potential was (86.31 ± 7.49) ms] (P <0.01), the positive area of cardiomyocytes [(19 193.38 ± 588.13) μm 2] and the number of positive cells [(18.50 ± 3.37) Group [(7 755.01 ± 586.16) μm2, (13.30 ± 1.49) / visual field] were significantly increased (P <0.05, P <0.01). Conclusions Serotonin is an inflammatory factor that leads to prolonged atrial action potential duration, increased heart rate and increased myocardial injury, and is also one of the many factors that cause cardiac dysfunction in sepsis.