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目前分析吞噬的方法,主要限于流式细胞仪和手动成像分析,提供的信息有限,无法实现高通量的快速筛选和吞噬定位。本文应用高速成像流式细胞仪这一以成像为基础的自动化分析系统,定量分析吞噬过程的结合和内化。结果显示双阳性信号的细胞中,约64%为吞噬了微珠的细胞,另有约36%为结合微珠的细胞。因此,运用成像流式细胞仪可以准确定量吞噬中真正发生内化的细胞,区别开处于结合状态的细胞,为研究者们提供了研究吞噬作用的高通量方法。
The current method for analyzing phagocytosis is mainly limited to flow cytometry and manual imaging analysis, which provides limited information to enable high-throughput rapid screening and phagocytosis. In this paper, an imaging-based automated analysis system using high-speed imaging flow cytometry is used to quantitatively analyze the combination and internalization of phagocytosis. As a result, about 64% of the double-positive cells showed the cells that had beaten the beads, and about 36% were the cells that bound the beads. Therefore, the use of imaging flow cytometry to accurately quantify phagocytosis in the real occurrence of internalization of cells, in a state of binding cells, providing researchers with high-throughput study of phagocytosis.