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目的研究胡黄连Picrorhiza kurrooa 2-C-甲基-D-赤藓糖-4-磷酸胞苷转移酶(MCT)基因特征并预测MCT结构与功能位点。方法以胡黄连MCT基因为研究对象,利用NCBI网站及生物信息学软件对其碱基分布、氨基酸组成、亲疏水性、保守区及二级结构和三级结构进行预测和分析;并对9个物种的MCT基因进行多重比对与进化分析。结果胡黄连MCT基因的mRNA序列长为1 216 bp(GenBank JQ991625.1),编码由399个氨基酸组成的蛋白质,该蛋白质相对分子质量为44 448.5,其中量最高的为Ser(10.0%);整个多肽中疏水性氨基酸占59.5%,平均疏水值为0.050;与丹参Salvia miltiorrhiza MCT氨基酸序列对比同源性最高,达99%。结论胡黄连MCT基因处于稳定状态,编码的蛋白为疏水性蛋白,在进化过程中是相对保守的,获得的保守区段序列信息为其他物种MCT基因的克隆奠定了基础。
OBJECTIVE: To study the genetic characteristics of Picrorhiza kurrooa 2-C-methyl-D-erythrose-4-phosphate cytidotransferase (MCT) gene and predict the structure and function of MCT. Methods The MCT gene of P. huanglian was taken as the research object. The base distribution, amino acid composition, hydrophilicity and hydrophobicity, conserved region, secondary structure and tertiary structure were predicted and analyzed by NCBI website and bioinformatics software. Nine species MCT gene multiple alignment and evolutionary analysis. Results The mRNA sequence of MCT gene was 1 216 bp (GenBank JQ991625.1), encoding a protein consisting of 399 amino acids with a relative molecular mass of 44 448.5. Serum (10.0% Peptide hydrophobic amino acids accounted for 59.5%, the average hydrophobic value of 0.050; and Salvia miltiorrhiza MCT amino acid sequence comparison of the highest homology, up to 99%. Conclusions The MCT gene of Hu Huanglian is in a stable state. The encoded protein is a hydrophobic protein that is relatively conserved during evolution. The obtained sequence information of the conserved region lays the foundation for the cloning of MCT gene from other species.