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芦笋茎枯病是一种世界性分布的毁灭性病害,为准确鉴定其致病菌和探明不同区域菌株的分化程度,通过形态学观察和核糖体DNA内转录间区(rDNA ITS)序列比对进行形态学和分子生物学鉴定,比较五省份菌株的生物学特性和ITS序列上的差异,并进行系统发育分析。结果表明,芦笋茎枯病的致病菌为天门冬拟茎点霉Phomopsis asparagi。海南省菌株的菌丝生长较快,培养5天后的平均直径为8.5 cm;培养14天后,江西省菌株由白色变为淡绿色,其它各省菌株由白色变为灰白色,海南省菌株的菌落呈现同心轮纹状;福建省菌株产生的分生孢子器较多,平均60个/皿;五省菌株在1～238 bp和298～591 bp的ITS区段存在差异性碱基,其中河北省菌株的差异性碱基数最多;五省菌株大致聚为2个组群,河北省菌株单独聚为1个组群,其它省份菌株聚为1个组群,天门冬拟茎点霉P.asparagi与叶下珠生拟茎点霉P.phyllanthicola亲缘关系较近。
Asparagus stem blight is a worldwide distribution of devastating diseases. In order to accurately identify the pathogenic bacteria and to determine the differentiation degree of the strains in different regions, the morphological observation and the rDNA ITS sequence ratio Morphological and molecular biological identification, comparison of five provinces strains of biological characteristics and ITS sequence differences, and phylogenetic analysis. The results showed that the pathogen of asparagus stem blight was Phomopsis asparagi. The mycelial growth of Hainan strains was rapid, with the average diameter of 8.5 cm after 5 days of culture. After 14 days of cultivation, the strains of Jiangxi Province turned from white to light green, and the strains of other provinces turned from white to off-white. The colonies of Hainan strains showed concentric There were more conidiospores in Fujian strains, with an average of 60 per pan. There were different bases in ITS regions of 1 ~ 238 bp and 298 ~ 591 bp in five provinces strains, among which, The number of different bases was the most. Five strains were clustered into two groups. The isolates in Hebei Province were clustered into one group separately. The strains in other provinces were clustered into one group. P.asparagi The lower parent Phyttrium P.phyllanthicola is closely related.