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目的以磺化-β-环糊精(S-β-CD)为手性选择剂,建立毛细管区带电泳法分离文拉法辛、奥昔布宁、维拉帕米、萘哌地尔、氟西汀对映体。方法采用未涂壁熔融石英毛细管柱,磷酸二氢钠缓冲溶液为背景电解质,分离电压为-15 kV,考察了环糊精质量浓度、缓冲溶液的pH值、缓冲盐浓度对对映体分离的影响。结果在最优的电泳条件下5种药物均达到基线分离。结论 S-β-CD是一种具有较强分离选择性的手性选择剂。
OBJECTIVE To establish a capillary zone electrophoresis method for the separation of venlafaxine, oxybutynin, verapamil, naftopidil, and pirarubicin using sulfonated β-cyclodextrin (S-β-CD) Fluoxetine enantiomer. Methods Uncoated fused silica capillary column and sodium dihydrogen phosphate buffer solution were used as the background electrolyte. The separation voltage was -15 kV. The effects of cyclodextrin concentration, buffer pH and buffer salt concentration on enantiomeric separation influences. Results Under the optimal electrophoresis conditions, all the five drugs achieved baseline separation. Conclusion S-β-CD is a selective chiral selector.