论文部分内容阅读
用考马斯蓝染色法显示了几种体外培养细胞的细胞骨架。在正常细胞中,如小鼠心肌细胞、小鼠食管上皮细胞以及人正常食管上皮细胞都有贯穿整个细胞并与细胞表面平行的纤维状结构。人食管癌上皮细胞中的纤维状结构少,而且排列不规则,形成网状。这些纤维状骨架受细胞松弛素D和高温(42.5℃与44.5℃)作用后呈弥散状。受美登木素或秋水仙素作用后仍然有部分存在,并有集合成索的现象。分裂细胞有蛋白质支架与其它细胞相连。讨论了用考马斯蓝显示的细胞骨架与肌动蛋白的关系。
Coomassie blue staining revealed several cytoskeletons of cultured cells in vitro. In normal cells, such as mouse cardiomyocytes, mouse esophageal epithelial cells, and human normal esophageal epithelial cells, there are fibrous structures throughout the cell and parallel to the cell surface. Human esophageal epithelial cells in the fibrous structure less, but irregular arrangement, the formation of reticular. These fibrous scaffolds were diffused by cytochalasin D and high temperature (42.5 ° C and 44.5 ° C). After the role of maytansinoids or colchicine is still part of the existence, and the phenomenon of a combination of cable. Split cells have a protein scaffold attached to other cells. The relationship between cytoskeleton and actin was demonstrated using Coomassie blue.