【摘 要】
：
[背景]金属硒化物因其优异的光电和催化特性,近年来在半导体、电化学及抗癌等领域成为了研究热点.相较于传统的化学还原法,生物合成金属硒化物具有环境友好、耗能较低等优势.然而,目前有关生物合成金属硒化合物的微生物资源较少且相关合成机理尚不明晰.[目的]利用马利亚霉菌(Mariannaea sp.) HJ合成了3种金属硒化物并对其合成机理进行了初步探索.[方法]利用X射线衍射(X-Ray Diffraction,XRD)和傅里叶转换红外线光谱(Fourier Transform Infrared Spectro
【机 构】
：
大连理工大学环境学院工业生态与环境工程教育部重点实验室 辽宁大连 116024
论文部分内容阅读
[背景]金属硒化物因其优异的光电和催化特性,近年来在半导体、电化学及抗癌等领域成为了研究热点.相较于传统的化学还原法,生物合成金属硒化物具有环境友好、耗能较低等优势.然而,目前有关生物合成金属硒化合物的微生物资源较少且相关合成机理尚不明晰.[目的]利用马利亚霉菌(Mariannaea sp.) HJ合成了3种金属硒化物并对其合成机理进行了初步探索.[方法]利用X射线衍射(X-Ray Diffraction,XRD)和傅里叶转换红外线光谱(Fourier Transform Infrared Spectroscopy,FTIR)对菌株HJ合成的金属硒化物进行了初步的表征,考察了纳米材料合成过程中总巯基含量、总抗氧化性能及自由基含量变化,并且验证了转运蛋白DMT1在金属硒化物合成中所起的关键性作用.[结果]XRD结果表明菌株HJ能够在Bi3+、Pb2+、Co2+与SeO32-作用下分别合成Bi4Se3、PbSe和CoSe2纳米颗粒,其合成的最优pH条件分别为6.0、7.0、8.0.FTIR结果表明,合成的金属硒化物表面含有氨基、羧基、羟基等官能团.3种金属硒化物的合成反应体系与空白对照组相比,总巯基含量明显下降,而总抗氧化性能却有所提高,这表明巯基等酶促体系或氨基酸金属蛋白类的非酶促体系可能参与了SeO32-的还原过程.苄基异硫脲盐酸盐屏蔽实验表明,转运蛋白DMT1在SeO32-转运和金属硒化物分泌过程中起到关键作用.此外,Bi3+、Pb2+和Co2+的加入使得菌株HJ产生氧化应激反应,在胞外分泌了大量的过氧化氢、羟基自由基和超氧自由基,而上述自由基可通过诱导热激效应的方式增强金属离子或纳米颗粒的转运过程.[结论]利用马利亚霉菌(Mariannaea sp.) HJ合成了Bi4Se3、PbSe和CoSe2纳米颗粒,为研究金属硒化物的生物合成及机理提供了一定的理论参考.
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