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据报道,疟疾传播能否阻断取决于特异性抗体的浓度和配子体的质量。为验证这一假设,作者等用抗配子和抗合子/动合子单克隆抗体,在不同条件下对这一问题作了探讨。将培养14天、内含成熟配子体的培养物置于3个锥形管,摇匀,预热离心后加0.5ml不含血清的无菌细胞,放在含碳酸氢盐的RPMI1640培养基内,用50%人红细胞悬液作5~6倍稀释,再以适量50%新鲜红细胞进一步稀释。这种不同稀释度的原虫培养液
It has been reported that whether malaria transmission can be blocked depends on the concentration of specific antibodies and the quality of gametocytes. To test this hypothesis, the authors investigated this issue under different conditions using anti-gametes and anti-integrin / actin monoclonal antibodies. After 14 days of culture, the culture containing the mature gametophyte was placed in three conical tubes and shaken. Preheated and centrifuged, 0.5 ml of sterile serum-free cells were placed in a bicarbonate-containing RPMI 1640 medium supplemented with 50% human erythrocyte suspension for 5 to 6 times diluted, then an appropriate amount of 50% fresh red blood cells for further dilution. This different dilution of protozoa broth