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目的应用核磁共振一维氢谱(1 HNMR)的代谢组学技术区分早期肾透明细胞癌(ccRCC)患者和健康人的血清代谢物差异。方法选取11例低级ccRCC患者(均为Ⅰ～Ⅱ级)和16例健康人的血清,用Bruker 600 MHz核磁共振谱仪采用cpmgpr1d脉冲序列并做单脉冲压制水峰检测1 HNMR。用主成分分析法(PCA)和偏最小二乘判别分析法(PLS-DA)分析血清代谢物的改变。结果结合PCA和PLS-DA统计方法,结果表明与健康人比较,低级ccRCC患者血清中的低密度脂蛋白(LDL)、极低密度脂蛋白(VLDL)、异亮氨酸、丙氨酸、N-乙酰糖蛋白(NAC)、乙酰乙酸、谷氨酰胺、牛磺酸、1-甲基组氨酸和苯丙氨酸的含量升高(P<0.05或P<0.01);乳酸、醋酸盐、丙酮酸、胆碱类物质、甘油和葡萄糖的含量降低(P<0.05或P<0.01)。结论 1HNMR技术能显著区分早期的ccRCC患者和健康人的血清代谢物差异,为ccRCC的早期诊断提供了一种新的有效方法。
OBJECTIVE: To identify the difference of serum metabolites between patients with early renal clear cell carcinoma (ccRCC) and healthy people by 1H NMR metabonomics. Methods Eleven patients with low grade ccRCC (grade Ⅰ ~ Ⅱ) and 16 healthy individuals were enrolled in this study. The 1H nuclear magnetic resonance spectroscopy (1HNMR) was performed on a Bruker 600 MHz nuclear magnetic resonance spectrometer using the pulse sequence of cpmgpr1d. Serum metabolites changes were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). Results Combined with PCA and PLS-DA statistical methods, the results showed that serum LDL, VLDL, isoleucine, alanine, N - NAC, acetylacetate, glutamine, taurine, 1-methylhistidine and phenylalanine (P <0.05 or P <0.01); lactic acid, acetate , Pyruvate, choline, glycerol and glucose (P <0.05 or P <0.01). Conclusion 1HNMR can significantly differentiate serum metabolites between early ccRCC patients and healthy individuals, providing a new and effective method for the early diagnosis of ccRCC.