微丝蚴固相抗原酶免疫试验诊断丝虫病的初步报告

• 作 者: 袁行政 沈丽英 邓珊珊 张素娥 裘祖祎
• 机 构: 浙江人民卫生实验院寄生虫病研究所,浙江人民卫生实验院寄生虫病研究所,浙江人民卫生实验院寄生虫病研究所,浙江人民卫生实验院寄生虫病研究所,浙江人民卫生实验院寄生虫病研究所
• 出 处: 上海免疫学杂志
• 发表日期: 1982年04期
• 关键词: 酶免疫试验 固相抗原 丝虫病 微丝蚴 微丝蚴血症 厚血膜片 可疑阳性 病人血清 假阳性 钩虫病

以微丝蚴固相抗原酶免疫试验法,检查了101例微丝蚴血症患者和200例健康人。按本文拟订的标准判断阳性、可疑阳性、假阴性和假阳性率分别为:91.09％,7.92％,0.99％和1.5％。在与血吸虫、肺吸虫和钩虫病人血清作交叉试验中,仅发现与钩虫病人(1/10)有交叉阳性。用本法和厚血膜片法现场检查了480例,发现前者阳性20例,后者7例(其酶免疫试验均阳性)。初步认为,本法诊断丝虫病具有较好的敏感性、特异性和重现性,因而具有一定实用价值。

To microfilaria solid phase antigen immunoassay, 101 cases of microfilaremia and 200 healthy people were examined. The positive, suspicious, false-negative and false-positive rates were 91.09%, 7.92%, 0.99% and 1.5%, respectively. In cross-tests with sera from patients with schistosoma, paragonimiasis and hookworm, only cross-positives were found with hookworm patients (1/10). Forty-eight cases were examined on-site using this method and the thick membrane method, and the former was found to be positive in 20 cases and the latter in 7 cases (the enzyme immunoassay was positive). Preliminary believe that the diagnosis of filariasis in this method has good sensitivity, specificity and reproducibility, which has a certain practical value.