论文部分内容阅读
以铝敏感型黑大豆(简称SB)为材料,研究铝胁迫下壳梭孢素(FC)对SB根铝耐受性的影响。结果表明:在50μmol·L-1铝胁迫下,添加1μmol·L-1FC处理8 h能够显著缓解铝胁迫对SB根生长的抑制作用,使其根的相对生长率(RRG)增加36.4%。免疫共沉淀分析结果表明50μmol·L-1铝胁迫下添加1μmol·L-1FC增强SB根中14-3-3蛋白与磷酸化质膜H+-ATPase的结合,使根尖质膜H+-ATPase活性提高约1倍,根氢泵活性和质子外排氢离子能力显著增强,根尖柠檬酸的分泌量增加显著,增加约2倍。这些结果证实铝胁迫下FC用于增强SB根中14-3-3蛋白和质膜H+-ATPase的相互作用和质膜H+-ATPase的活性,从而增加SB根柠檬酸的分泌作用及其对铝胁迫的耐受性。
Aluminum-sensitive black soybean (SB) was used as material to study the effects of endosymbionts (FC) on aluminum tolerance of SB roots under aluminum stress. The results showed that under the stress of 50μmol·L-1 aluminum, the root growth rate (RRG) increased by 36.4% after being treated with 1μmol·L-1FC for 8 h. Co-immunoprecipitation analysis showed that the addition of 1μmol·L-1FC under 50μmol·L-1 aluminum stress enhanced the binding of 14-3-3 protein to H + -ATPase in SB root, and the activity of H + -ATPase in root apical membrane Increased by about 1 time, root hydrogen pump activity and proton excretion ability was significantly enhanced root tip citric acid secretion increased significantly, an increase of about 2 times. These results demonstrate that FC is used to enhance the interaction of 14-3-3 protein with plasma membrane H + -ATPase and plasma membrane H + -ATPase activity in SB roots under aluminum stress, thereby increasing the secretion of citrate from SB root and its effect on aluminum Stress tolerance.