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目的用红外光谱方法分析氟对实验动物亚细胞结构的影响。方法将32只无特定病原体级纯种昆明小鼠随机分为低氟、中氟、高氟3个染氟组和对照组,染氟组分别给予腹腔注射氟化钠2、8、32 mg.(kg.d)-1,对照组注射等体积生理氯化钠,连续14 d。末次注射后24 h处死动物,分离肝细胞的细胞核、线粒体和微粒体3种亚细胞结构作傅里叶红外光谱(ATR-FTIR)分析。结果与对照组比较,各染氟组3种亚细胞结构在1 650～930 cm-1各峰值均降低(P<0.05),呈剂量-效应关系,敏感度依次为:微粒体>线粒体>细胞核;主成分分析显示:染氟使细胞核化学成分磷酸化增加、线粒体及微粒体多种化学成分改变。结论本实验条件下,ATR-FTIR检测到氟致肝细胞核、线粒体、微粒体的化学成分发生改变;ATR-FTIR有望作为细胞毒性反应的早期监测方法之一。
Objective To analyze the effect of fluorine on the subcellular structure of experimental animals by infrared spectroscopy. Methods Thirty-two non-specific pathogen-grade Kunming mice were randomly divided into three groups: low fluoride, medium fluoride and high fluoride, and control group. The rats in the fluoride group received intraperitoneal injections of sodium fluoride at 2, 8 and 32 mg respectively. (kg.d) -1. The control group was injected with equal volume of physiological sodium chloride for 14 days. Animals were sacrificed at 24 h after the last injection, and three subcellular structures of nucleus, mitochondria and microsome of hepatocytes were isolated for ATR-FTIR analysis. Results Compared with the control group, the three kinds of subcellular structures of each fluoride group decreased in the range of 1 650-930 cm-1 (P <0.05), showing a dose-response relationship with the following sensitivities: microsomes> mitochondria> nuclei The principal component analysis showed that fluoride exposure increased the phosphorylation of chemical components of the nucleus and changed the chemical components of mitochondria and microsome. CONCLUSIONS ATR-FTIR has been shown to change the chemical composition of liver nuclei, mitochondria and microsomes induced by fluoride in vitro. ATR-FTIR is expected to be one of the early monitoring methods for cytotoxicity.