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以中华芦荟的根、茎、叶为外植体 ,探讨了诱导的愈伤组织与芦荟素积累的关系。分别用薄层色谱法和高效液相色谱法测定。结果表明 ,在MS +NAA 1mg/L + 6 BA 0 5mg/L培养基上 ,以叶为外植体产生的愈伤组织 ,分化程度最高 ,芦荟素含量也高 ( 0 35 % ) ;以茎为外植体产生的愈伤组织 ,芦荟素含量次之 ( 0 0 8% ) ;以根为外植体产生的愈伤组织不含芦荟素。在MS + 2 ,4 D 1mg/L + 6 BA 0 5mg/L培养基上 ,以根、茎、叶为外植体产生的愈伤组织 ,分化程度低 ,均不含芦荟素
The roots, stems and leaves of Aloe chinensis were used as explants to investigate the relationship between the induced callus and the accumulation of aloin. Determined by thin layer chromatography and high performance liquid chromatography, respectively. The results showed that on the medium of MS +NAA 1mg/L + 6 BA 0 5mg/L, callus produced from leaves as explants had the highest degree of differentiation, and the content of aloin was also high (0.35%); The callus produced by explants was followed by alovain content (0 0 8% ); callus produced from roots as explants did not contain aloin. In the medium of MS +2,4 D 1mg/L + 6 BA 0 5mg/L, roots, stems and leaves were used as explants to produce callus with low degree of differentiation.