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为了研究热带西太平洋深海沉积物中古菌群落结构的多样性,应用16S rDNA文库技术,对IMAGESⅪⅤ航次采集的岩芯MD3059进行基因文库的构建和分析,选取得到543个克隆,处理获得137个OTUs(Operational Taxonomic Units)。基于16S rRNA序列的同源性比较,绘制系统进化树,进行统计学分析。研究结果显示,热带西太平洋苏禄海沉积物中有丰富多样的古菌群落,在不足5m的沉积物中垂直分布着11种古菌类群。古菌序列归属于泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),其中,前者主要由Miscellaneous Crenarchaeotic Group(MCG)构成(占总古菌克隆序列的50%以上),而后者主要由Marine Benthic Group D(MBG-D)、South Africa Gold Mine Eu-ryarchaeotic Group(SAGMEG)和Marine Benthic Group B(MBG-B)等种属构成(占总古菌克隆序列的30%以上)。研究结果对于理解热带西太平洋深海沉积物中微生物多样性和未来开发利用微生物资源具有重要意义。
In order to study the diversity of archaeal community structure in deep-sea sediments of the tropical western Pacific, 16S rDNA library technology was used to construct and analyze the gene library of core MD3059 collected from IMAGES XIV voyage. 543 clones were selected and 137 OTUs Operational Taxonomic Units). Based on the homology comparison of 16S rRNA sequences, phylogenetic tree was drawn for statistical analysis. The results show that there are rich and varied archaeal communities in the sediments of the Sulu Sea in the tropical western Pacific, and 11 archaeal groups are vertically distributed in sediments of less than 5m. The archaeal sequences are attributed to Crenarchaeota and Euryarchaeota, of which the former consists predominantly of the Miscellaneous Crenarchaeotic Group (MCG) (over 50% of the total archaeal clonal sequences), while the latter is mainly composed of Marine Benthic Group D (MBG-D), South Africa Gold Mine Eu-ryarchaeotic Group (SAGMEG) and Marine Benthic Group B (MBG-B) (accounting for more than 30% of total archaeal cloning sequences). The results of this study are of great importance for understanding the diversity of microorganisms in deep-sea sediments of the tropical western Pacific and the future development and utilization of microbial resources.