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本研究表明:无论有无NADPH的存在,苯巴比妥钠预处理均可使大鼠肝匀浆中甲醇代谢率明显增加,而SKF—525—A预处理则无效。在体外测试系统中加入叠氮化钠可明显抑制甲醇代谢,而加入NADPH则可增加其代谢。相反,SKF—525—A在这方面无任何作用。 甲醇代谢的酶动力学研究表明:Km和Vmax分别为4.4mM和1.1nM/mg蛋白/分。当反应体系中加入2.5mM和12.5mM乙醇时,Km值分别变为6.7mM和11.4mM,而Vmax不变。然而,当加入1.0mM叠氮化钠时,Vmax减少而Km增加。
This study shows that: with or without the presence of NADPH, pretreatment with phenobarbital can significantly increase the methanol metabolism rate in rat liver homogenate, while SKF-525-A pretreatment is ineffective. Addition of sodium azide to an in vitro test system significantly inhibits methanol metabolism, whereas addition of NADPH increases its metabolism. In contrast, SKF-525-A has no effect in this regard. Enzyme kinetic studies of methanol metabolism showed that Km and Vmax were 4.4 mM and 1.1 nM / mg protein / min, respectively. When 2.5 mM and 12.5 mM ethanol were added to the reaction system, the Km values became 6.7 mM and 11.4 mM, respectively, while the Vmax did not change. However, when 1.0 mM sodium azide was added, Vmax decreased and Km increased.