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目的研究哮喘动物气道细胞的DNA合成和气道重塑以及二者之间的关系。方法建立大鼠哮喘模型,采用双标免疫组化技术和计算机图像分析系统对气道细胞的DNA合成和气道重塑进行研究。结果 （1）哮喘组气道平滑肌细胞和上皮细胞的DNA合成BrdU阳性计数明显高于对照组（P<0.01,P<0.05）;（2）哮喘组气道平滑肌细胞和上皮细胞DNA合成Brdu阳性计数和气道直径呈高度正相关（P<0.01,P<0.05）,对照组无明显相关性（P<0.05,P<0.05）;（3）哮喘组气道上皮层厚度明显大于对照组（P<0.01）,平滑肌层厚度、气道直径和α-平滑肌肌动蛋白阳性区面积和对照组无显著性差异（P>0.05）。结论变态反应性炎症所导致的气道细胞DNA合成增加以及细胞增生,可能是引起气道重塑反应发生的重要原因。
Objective To study the DNA synthesis and airway remodeling of asthmatic airway cells and their relationship. Methods A rat model of asthma was established. Double-labeled immunohistochemistry and computer image analysis system were used to study the DNA synthesis and airway remodeling of airway cells. Results (1) The BrdU positive counts of DNA synthesis in asthmatic airway smooth muscle cells and epithelial cells were significantly higher than those in control group (P <0.01, P <0.05). (2) Brdu positive in DNA synthesis of airway smooth muscle cells and epithelial cells in asthma group There was a positive correlation between count and airway diameter (P <0.01, P <0.05), but there was no significant correlation between the count and airway diameter (P <0.05, P <0.05). (3) The thickness of airway epithelium in asthma group was significantly greater than that in control group 0.01). There was no significant difference in the thickness of smooth muscle layer, airway diameter and α-smooth muscle actin positive area between the two groups (P> 0.05). Conclusion The increase of DNA synthesis and cell proliferation in airway cells caused by allergic inflammation may be the important reason of airway remodeling.