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本实验旨在测定海群生治疗后循环微丝蚴数量减少对血清微丝蚴抗体滴度的影响。实验是在菲律宾卡坦端内斯省进行的。当地为夜间周期型班氏丝虫病的高度流行区。8例无慢性淋巴管阻塞的微丝蚴血症者志愿参加实验,其中5例男性,3例女性,年龄为12～77岁。每一患者口服海群生10mg/kg/天,共3周。治前与治后1、2、3、6周晚9时采集血标本,计数经核孔过滤的1ml抗凝血中的微丝蚴数。并收集血清以测定微丝蚴凝集抗体:从感染沙鼠的腹腔取得马来丝虫微丝蚴,经Eagle氏磷酸盐缓冲液洗涤后稀释至每ml含1,000条微丝蚴,加至置
The purpose of this experiment was to determine the effect of reducing the number of circulating microfilariae on the titer of serum microfilariae after treatment of seaquot. The experiment was conducted in Catanduanes Province, Philippines. The local area is a highly endemic area of Bancroftian filariasis at night. 8 cases of non-chronic lymphatic obstruction microfilaremia who volunteered to participate in the experiment, including 5 males and 3 females, aged 12 to 77 years. Each patient oral herds of 10mg / kg / day, a total of 3 weeks. Blood samples were collected before and after treatment at 1, 2, 3, 6 weeks after treatment at 1, and the number of microfilariae in 1 ml anticoagulant filtered by nuclear was counted. And the sera were collected for the determination of microfilariae agglutination antibodies: Filamentous microfilaria was obtained from the abdominal cavity of gerbils infected with gerbils, washed with Eagle’s phosphate buffer and diluted to 1,000 microfilaments per ml,