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以3例红细胞内期形态符合间日疟原虫多核亚种感染的病人为血源,通过羊膜饲血法离体感染和直接叮咬自愿者感染中华按蚊,在26±1℃相对湿度为70～80％的恒温室分笼饲养。自按蚊吸血后5分钟至40小时,按予定时间解剖蚊胃,做胃血薄涂片,观察大、小配子及动合子的形成;自第3～11天,
Three cases of erythrocytic end stage conformal infection of P. vivax multinucleus were used as the blood source, and infected with Anoectochilus sinensis in vitro by direct inoculation of amniotic fluid and direct bites. The relative humidity at 26 ± 1 ℃ was 70 ~ 80% of the greenhouse room kept in cage. From anopheles mosquito blood 5 to 40 hours after the scheduled time to dissect the mosquito stomach thin stomach smear to observe the formation of large and small gametes and zygotes; from the first 3 to 11 days,