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为了加速湿地松(Pinus elliottii Engelm.)苗木繁殖,湖北荆州地区林科所于1973年开始以短枝进行水培育苗,经过不断改进,目前生根率已达80—90％,突破了生产难关。本文试就其水培过程中愈伤组织及不定根的形成作细胞组织学的探讨。一、材料及方法样品由荆州地区林科所提供,方法为采取1—2年生湿地松苗木上的当年生短枝(叶束),以锋利刀片切平短枝基部,每50根扎成一小捆,经沙藏一周后,以100ppm或200ppm的萘乙酸水溶液浸泡基部一昼夜,再置于盛有50毫升基本培养液的玻璃容器中(基本培养液配方:硼酸50ppm,维生素B_1,硝酸铵20ppm),在塑料棚温室内温度控制在18—28℃
In order to speed up Pinus elliottii Engelm. Seedling propagation, Linzhou, Hubei Jingzhou region in 1973 began to short sprouting water cultivation, after continuous improvement, the current rooting rate has reached 80-90%, breaking the production difficulties. This paper try to hydroponic process callus and adventitious root formation for cell histology. I. MATERIALS AND METHODS Samples were provided by the Linke Institute of Jingzhou Prefecture by cutting the annual shoots (leaves bundles) of 1 to 2 year old wetland pine seedlings, cutting the base of short branches with sharp blades, After one week of storage in sand bank, the basal body was soaked in 100ppm or 200ppm naphthalene acetic acid for one day and night, and placed in a glass container containing 50ml basic medium (basic medium solution: boric acid 50ppm, vitamin B_1, ammonium nitrate 20ppm) , In greenhouse plastic greenhouse temperature control at 18-28 ℃