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水中的阴离子表面活性剂难以降解，在水体中容易聚集，而恶化水质。为了建立一种更加简单、快速、准确的测定水中阴离子表面活性剂的方法，对亚甲蓝分光光度法和连续流动分光光度法两种方法进行比较。阴离子染料亚甲蓝易与水样中阴离子合成活性剂发生作用形成蓝色盐类（MBAS），用氯仿萃取后分别用两种方法测定比较结果。结果显示：两种检验方法测定结果并无显著差异，连续流动分光光度法灵敏度较高且可以自动、便捷、大量测得数据。“,”Anionic surfactants in water are difficult to degrade and aggregate easily in water, which deteriorates the water quality. rnIn order to establish a more simple, rapid and accurate method for the determination of anionic surfactants in water, methylene blue spectrophotometry and continuous flow spectrophotometry were compared. Anionic dye methylene blue easily reacts with anionic synthetic activator in water to form blue salts (MBAS). The results were determined by two methods after chloroform extraction. The results show that there is no significant difference between the two methods. Continuous flow spectrophotometry has high sensitivity and can automatically, conveniently and quantitatively measure data.