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目的 观察重组日本血吸虫 2 6kDaGST抗原 (reSjc2 6GST)免疫役用放养水牛 (简称水牛 )后抗体动态及免疫保护性的效果。 方法 试验组 96头水牛 ,用reSjc2 6GST免疫 3次 ,每次间隔 2wk ,3次剂量分别为 0 2、 0 2和 0 1mg。对照组 90头水牛不作免疫 ;观察 2组水牛免疫前及免疫后 2、 5、 9、 12、 15和 2 0个月的抗体水平及血吸虫感染率的变化。 结果 试验组机体产生特异性抗reSjc2 6GST抗体 ,其抗体水平呈明显的梯形升高趋势。试验组免疫后 2 0个月血吸虫感染率比免疫前下降了 62 2 % ,比同期对照组低 67 7%。 结论 用reSjc2 6GST免疫水牛能产生特异性抗体 ,在免疫后 2 0个月内维持较高水平 ,有一定的抗血吸虫自然感染的保护力。
Objective To observe the antibody activity and immunoprotective effect of reSjc2 6GST immunized with buffalo buffalo after immunization. Methods Ninety-six buffaloes were immunized with reSjc2 6GST three times at intervals of 2 weeks and the three doses were 0 2, 0 2 and 0 1 mg, respectively. The control group of 90 buffaloes were not immunized; the antibody levels and the infection rate of schistosomes were observed before immunization and at 2, 5, 9, 12, 15 and 20 months after immunization. Results The experimental group produced specific anti-reSjc2 6GST antibody, the antibody level showed a significant trapezoidal rise. The infection rate of schistosomiasis in experimental group decreased 62 62% after immunization compared with that before immunization, 67 67% lower than the control group at the same period. Conclusion ReSjc2 6GST immune buffaloes can produce specific antibodies, maintain a high level within 20 months after immunization, have a certain degree of natural protection against infection by schistosome.