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目的观察γ链细胞因子在艾滋病病毒(HIV)感染者/艾滋病(AIDS)病人(HIV/AIDS病人)和正常人表达水平的区别,及其在24个月的高效抗反转录病毒治疗(HAART)治疗中的动态变化,分析其与抗病毒治疗的关系及其临床意义。方法 40名HIV/AIDS病人在初次HAART治疗的0、6、12、18和24个月进行回访,另35名正常人作为对照,均采集外周血,用酶联免疫吸附试验(ELISA)检测常见的γ链细胞因子[白细胞介素2、4、7、15、21(IL-2、IL-4、IL-7、IL-15、IL-21)和γ干扰素(IFN-γ)],同时使用流式细胞计数法测定CD4+T和CD8+T淋巴细胞数量,实时反转录聚合酶链反应(RT-PCR)法测定血清中HIV核糖核酸(RNA)水平。结果未经抗病毒治疗的HIV/AIDS病人外周血IL-2、IL-15、IL-21和IFN-γ水平显著低于正常人,而IL-4和IL-7水平显著高于正常人。在启动HAART后的各次回访中,随着外周血HIV RNA病毒载量水平和CD8+T淋巴细胞数量的逐渐下降和CD4+T淋巴细胞数量的显著增加,IL-2、IL-15、IL-21和IFN-γ水平也逐步上升,而IL-4和IL-7水平逐步下降,显示出一定的相关关系。结论这些常见的γ链细胞因子可能和HIV感染的致病机制相关,并可能参与了抗病毒免疫重建和病毒控制过程。
Objective To observe the difference in the expression of γ chain cytokines in HIV / AIDS patients (normal people) and their HIV / AIDS patients (24 h) ) In the treatment of dynamic changes, analysis of its relationship with antiviral therapy and its clinical significance. Methods A total of 40 HIV / AIDS patients were interviewed at 0, 6, 12, 18 and 24 months after the initial HAART treatment. Another 35 normal people were used as control. Peripheral blood samples were collected and detected by enzyme-linked immunosorbent assay (ELISA) [Interleukin 2, 4, 7, 15, 21 (IL-2, IL-4, IL-7, IL-15, IL-21 and IFN- [gamma] At the same time, the number of CD4 + T and CD8 + T lymphocytes was measured by flow cytometry and the level of HIV RNA in serum was determined by real-time reverse transcription-polymerase chain reaction (RT-PCR). Results The levels of IL-2, IL-15, IL-21 and IFN-γ in peripheral blood of HIV / AIDS patients without antiviral therapy were significantly lower than those in normal people, while the levels of IL-4 and IL-7 were significantly higher than those in normal people. In each visit after starting HAART, IL-2, IL-15 and IL were increased with the decrease of HIV RNA viral load and the number of CD8 + T lymphocytes in peripheral blood and the significant increase of CD4 + T lymphocytes -21 and IFN-γ levels also gradually increased, while IL-4 and IL-7 levels gradually decreased, showing a certain correlation. Conclusion These common γ-chain cytokines may be involved in the pathogenesis of HIV infection and may be involved in anti-viral immune reconstitution and virus control.