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用成年雄性Wistar大鼠65只,分溃疡实验组、盐水对照组和空白对照组。在无菌条件下,将0.01 ml冰醋酸注入动物胃粘膜下层,造成实验性胃溃疡;盐水组模拟手术,注入0.01ml生理盐水;空白组为未经任何处理的正常大鼠。3组动物在相同条件下饲养,在手术后2～28d,分7批取材,取出胰尾进行酶组织化学观察。盐水组:A细胞在手术后2～4 d,AlP活性减弱,5～Nase活性增强,10 d恢复;B细胞在手术后2～4 d,AcP及5-Nase活性增强,ATPase活性减弱,10 d基本恢复。溃疡组:A细胞在手术后2～4d酶活性变化与盐水组相同。手术后6d,AlP、ATPase、SDH、LDH、G-6-PD及α-GPD活性均显示出不同程度增强,至手术后28 d恢复;B细胞在手术后21 d的变化与盐水组基本一致,但手术后4 d,AcP、5-Nase、SDH及LDH活性均减低,21 d恢复。本文实验结果提示,在大鼠实验性胃溃疡期间,胰岛A、B细胞共同参与了机体自然抗病过程。
Sixty-five adult male Wistar rats were used to divide the ulcer test group, the saline control group, and the blank control group. Under aseptic conditions, 0.01 ml of glacial acetic acid was injected into the submucosa of the stomach of the animal to cause experimental gastric ulcer; in the saline group, surgery was performed and 0.01 ml of physiological saline was injected; and the blank group was normal rats without any treatment. The three groups of animals were reared under the same conditions. After 2 to 28 days after surgery, 7 batches were taken and the pancreatic tail was removed for enzyme histochemical observation. In the saline group, the activity of AlP decreased, the activity of 5-Nase increased, and the activity of 5-Nase increased in A cells at 2 to 4 days after surgery. The activity of AcP and 5-Nase increased and the activity of ATPase decreased in B cells at 2 to 4 days after surgery. d Basic recovery. In the ulcer group, changes in enzyme activity in A cells at 2 to 4 days after surgery were the same as in the saline group. At 6 days after surgery, the activities of AlP, ATPase, SDH, LDH, G-6-PD and α-GPD all showed different degrees of enhancement and recovered 28 days after surgery. The changes of B cells at 21 days after surgery were basically consistent with those of saline group. However, the activity of AcP, 5-Nase, SDH, and LDH decreased at day 4 after surgery, and recovered at 21 days. The experimental results in this paper suggest that during experimental gastric ulcers in rats, islet A and B cells are involved in the natural resistance process of the body.