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正常人周围血中的巨噬细胞(Mφ)吞噬麻风菌后,能释放一定量的反应性含氧中间介质(ROI),如超氧化物(O_2~-)和羟基(OH·)以杀死细菌。麻风病人的Mφ产生的ROI不足以杀死麻风菌。作者用脱脂后的麻风菌不溶成份(DCC,很可能是菌壁蛋白质)与患者周围血中的单核细胞共同孵育,其上清液加入同一患者的巨噬细胞培养液中能纠正巨噬细胞的这种缺陷。首先制备DCC刺激悬液。取犰狳组织的麻风菌,用生理盐水洗涤两次,调整细胞至10~(10)～10~(11)个/5ml,经超
Macrophage (Mφ) in peripheral blood of normal people can release a certain amount of reactive oxygen intermediates (ROI) such as superoxide (O 2 -) and hydroxyl (OH ·) to kill bacterial. The leftover Mφ produces insufficient ROI to kill Leprosy. The authors co-incubated with defatted leprosy insoluble components (DCC, most likely wall protein) with mononuclear cells in the patient’s peripheral blood, and the supernatant was added to macrophage cultures of the same patient to correct macrophages This defect. DCC stimulation suspensions were prepared first. Leprosy tissue taken armadillo tissue, washed twice with saline, adjust the cells to 10 ~ (10) ~ 10 ~ (11) / 5ml, the super