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在１９８７年分离、培养４株红豆草根瘤菌（红１、红２、红３、红４）的基础上，又分离出红三叶、鹰嘴紫云英、斜茎紫花黄芪、箭舌豌豆及美国Ｎｉｔｒｏ苜蓿根瘤菌５个菌株；对已回接成功的红豆草根瘤菌株“红１”和待回接菌株“红２”及红三叶、美国Ｎｉｔｒｏ苜蓿根瘤菌进行植株感染试验。其中“红１”菌株感染力有所降低，“红２”、“红三叶”及Ｎｉｔｒｏ苜蓿菌株感染植株均获成功。从感染后所结根瘤的形态解剖特征看，均属有效根瘤，并从红豆草感染植株的根瘤中分离到与接种菌株特性相同的根瘤菌株。普通砂培时，红豆草根瘤固氮活性与近年来所测苗期固氮活性一致。田间接菌试验新、老区结果不同。
On the basis of separating and cultivating 4 rhizobia of red bean (red 1, red 2, red 3 and red 4) in 1987, the red clover, the oyster millet, the astragalus membranaceus, the arrow tongue pea And five Nitro alfalfa strains in the United States. The plant infection test was carried out on Rhizobium japonicus strain “Red 1” and the bacterium “Red 2” and red clover which were successfully connected back to the United States. Among them, the infectivity of “Red 1” strain was reduced, and “Red 2”, “Red Clover” and Nitro alfalfa strains were all successfully infected. From the morphological anatomy of nodules after infection, it is an effective root nodule. Rhizobium strains with the same characteristics as inoculated strains were isolated from nodules infected with saponins. In common sand culture, the activity of nitrogen fixation in the root nodules of sainfoin is consistent with the nitrogen fixation activity measured in seedling stage in recent years. New field trials of bacteria inoculation, the old district results are different.