论文部分内容阅读
目的分析2009-2015年梧州市海水产品和水样霍乱监测结果,为进一步采取预防控制措施提供依据。方法根据《广西霍乱监测方案》和《霍乱防治手册》(第五版、第六版),对海水产品和水样进行采样监测、分离和鉴定。结果2009-2015年梧州市共监测海水产品和水样2 877份,阳性71份,总阳性率2.47%;其中海水产品监测1 790份,阳性55份,阳性率3.07%;水样监测1 087份,阳性16份,阳性率1.47%;海水产品和水样阳性率差异有统计学意义(χ2=7.199,P=0.007);不同样品中以蛙类标本检出阳性率最高,阳性率高达13.01%;3个城区阳性率差异有统计学意义(χ2=9.816,P=0.007);71株阳性标本中,O1群稻叶型34株,小川型30株,O139群6株,非O1群非O139群1株;71份菌株均为非产毒株,霍乱肠毒素(CT)毒力基因均为阴性,18份小带联结毒素(Zot)毒力基因阳性,阳性率25.35%。结论梧州市外环境存在适宜霍乱弧菌生长的因素,海水产品存在外源与本土2种情况污染,应加大对海水产品尤其是蛙类的监测力度,强化市场准入制度,避免霍乱疫情的发生和流行。
Objective To analyze the monitoring results of seawater products and water cholera from 2009 to 2015 in Wuzhou and provide the basis for further prevention and control measures. Methods Sampling, monitoring, separation and identification of marine products and water samples were conducted according to the “Cholera Surveillance Program in Guangxi” and the “Cholera Prevention and Control Manual” (Fifth and Sixth Editions). Results A total of 2 877 marine products and water samples were detected in Wuzhou from 2009 to 2015, with 71 positives and 2.47% positives. Among them, 1 790 were detected for marine products, 55 were positive and the positive rate was 3.07%. Water samples were monitored 1 087 (Χ2 = 7.199, P = 0.007). The positive rate of frogs was the highest in all samples, the positive rate was 13.01 %. There were significant differences in the positive rates of the three urban areas (χ2 = 9.816, P = 0.007). Among the 71 positive samples, 34 were O1 rice leaves, 30 were Ogawa, 6 were O139, O139 group. One hundred and seventy strains were non-toxigenic. The virulence genes of Cholera toxin (CT) were negative, and the virulence genes of 18 Zot strains were positive. The positive rate was 25.35%. Conclusion There are some factors that may affect the growth of Vibrio cholerae in the external environment of Wuzhou City. There are two kinds of pollution of seawater products exogenous and native, so it is necessary to strengthen the monitoring of marine products, especially frogs, strengthen market access system and avoid the epidemic of cholera Occurred and popular.