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目的应用微循环研究方法和分子生物学实验技术研究心肌细胞的生理功能指标，为寻找新的治疗靶点、筛选合理药物、防治心血管疾病奠定理论基础。方法培养新生大鼠心脏心尖、心房、左心室、右心室和室间隔五个部位的心肌细胞，用Ｔｉｍｅｒ Ｌａｐｓｅ Ｒｅｃｏｒｄｗｅ８０５０缩时录像技术和我所研制的微循环图像数学处理技术（ＭＣＩＰ）记录分析培养的心脏不同部位心肌细胞的功能指标、以便用分子生物学技术对心肌细胞的增殖、凋亡、受体表达和信号传导途径作进一步探索。结果通过正常的放像速度观察，发现心脏不同部位的心肌细胞，表现出特异性的形态学差别，并处于动态节律性搏动的状态。镜下可见聚集在一起的心肌细胞以不相同的搏动频率持续跳动，井向四周伸出伪足。本实验共检测１２３个心肌细胞，平率搏动范围在２９～１７５次／分、各部位细胞的搏动频率分别为心房７５．３０±１５． ３３次／分，右心室 ８４． １１± ２３． ４６次／分，室间隔 ９１． １５±３８．９２次／分，左心室 １０１．５７±１２．９４次／分，心尖 １１１．５３±１３． ９３次／分，总平均搏动频率为 ９０． ５３±２５． ５６次／分。结论上述实验结果表明，不同部位的心肌细胞其搏动频率不同，心尖部位细胞最快
Objective To study the physiological functions of cardiomyocytes by using microcirculation research methods and molecular biology experimental techniques to lay a theoretical foundation for finding new therapeutic targets, screening rational drugs and preventing and treating cardiovascular diseases. Methods Cardiac myocytes in apex, atrium, left ventricle, right ventricle and interventricular septum of neonatal rat heart were cultured and recorded using Timer Lapse Recordwe 8050 time-lapse recording technique and microcirculation image processing technique (MCIP) Cardiac function of different parts of the heart function index, in order to use molecular biology techniques on cardiomyocyte proliferation, apoptosis, receptor expression and signal transduction pathways for further exploration. The results of normal playback rate observed, found in different parts of the heart of myocardial cells, showing specific morphological differences, and in the dynamic rhythm of beating state. Microscopically, the cardiomyocytes that congregated together continued to beat at different pulsation frequencies, and pseudopods extended to the periphery. A total of 123 cardiomyocytes were detected in this experiment, the rate of beating at 29 ~ 175 beats / min, and the beating frequency of each part were 75.30 ± 15 atria. 33 beats / min, right ventricle 84. 11 ± 23. 46 beats / min, ventricular septum 91. 15 ± 38.92 beats / min, 101.57 ± 12.94 beats / min in the left ventricle, and 111.53 ± 13 in the apex. 93 beats / min, the total average beat frequency of 90. 53 ± 25. 56 beats / min. Conclusions The above experimental results show that the myocardial cells in different parts have different pulsation frequency and the fastest apex cells