【摘 要】
：
The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance, which is of great potential in cellulose degradation in high
【机 构】
：
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Bio
论文部分内容阅读
The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance, which is of great potential in cellulose degradation in high salt environment. In order to explain the mechanism on the salt-tolerance of the cellulase cocktail produced by marine A. niger, six cellulase components (AnCel6, AnCel7A, AnCel7B, AnEGL, AnBGL1 and AnBGL2) were obtained by directed expression. Studies on their enzymatic proper-ties revealed that oneβ-glucosidase (AnBGL2) and one endoglucanase (AnEGL) exhibited an outstanding salt-tolerant property, and one cellobiohydrolase (AnCel7B) exhibited a certain salt-tolerant property. Subsequent study revealed that the salt-tolerant AnEGL and AnCel7B endowed the cellulase cocktail with stronger salt-tolerant property, while the salt-tolerant AnBGL2 had no positive effect. Moreover, after overexpression of AnCel6, AnCel7A, AnCel7B and AnEGL, the activity of cellulase cocktail increased by 80％, 70％, 63％and 68％, respec-tively. However, the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2. After mixed-strain fermentation with cellobiohydrolase recombinants (cel6a, cel7a and cel7b recombinants) and endoglucanase recombinant (egl recombinant), the the activity of cellulase cocktail increased by 114％, 102％and 91％, respectively.
其他文献
In this work, a trickle-bed reactor coupled with catalyst pellet model is employed to understand the effects of the temperature and catalyst pellet structures o
目的： 非编码RNA（non-coding RNA，ncRNA）在细菌应对环境变化和致病过程中起重要作用。本课题组前期对伤寒沙门菌进行转录组测序分析发现了一系列的ncRNA，其中一个ncRNA，为bax的
This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae (Novozym 51003? laccase) on amino modified fum
In this work, Fe/Ni nanoparticles were produced through Fe(Ⅱ) and Ni(Ⅱ) reduction by NaBH4 and they were sta-bilized by a kind of prepared granular adsorbent
The hydrodynamics is still not fully understood in the three-phase stirred tank equipped with multi-impeller due to the intensive interaction between phases. In
This research investigates a numerical simulation of swirling turbulent non-premixed combustion. The effects on the combustion characteristics are examined with
目的：应用实时荧光定量PCR技术检测大鼠肌肉挫伤后骨骼肌肌钙蛋白Ⅰ(skeletaltroponinⅠ，sTnI)mRNA表达，探寻其时序性表达规律，探讨sTnImRNA表达变化应用于损伤时间推断的可行性，
The potentials of silty clay (SC), acquired from Chaman, Balochistan, were investigated as adsorbent for Ni (Ⅱ) and Cd (Ⅱ) removal from contaminated media. Th
Amine-based carbon dioxide (CO2) capture is still limited by high desorption energy consumption. Fixing CO2 into carbonate is a safer and more permanent method.
目的：研究十全大补汤总多糖的免疫抑瘤作用。通过动物实验观察不同剂量十全大补汤总多糖成分对荷瘤小鼠体内肿瘤的生长、脾脏T细胞和B细胞的增殖及其产生IL-2、IFN等细胞因子