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目的:研究三七总皂苷对模型小鼠上呼吸道黏膜免疫分子分泌型免疫球蛋白A(SIgA)调节作用。方法:通过腹腔注射青霉素建立菌群失调模型,观察三七总皂苷对小鼠呼吸道黏膜免疫分子SIgA调节作用影响。实验分3组:空白(阴性对照)组、青霉素模型组、三七总皂苷组。结果:小鼠在给予青霉素腹腔注射3d后,甲型链球菌、菌群密集度、菌群多样性均显著降低,提示模型复制成功。三七总皂苷能够显著升高因青霉素致菌群失调模型小鼠而降低的上呼吸道黏膜免疫分子SIgA。结论:青霉素致小鼠上呼吸道菌群失调模型可用于中医药调节小鼠上呼吸道感染的研究;三七总皂苷预防和治疗上呼吸道感染的作用机制之一是促进上呼吸道黏膜免疫分子SIgA的分泌,增强上呼吸道黏膜的局部免疫作用。
Objective: To study the regulation effect of Panax notoginseng saponins on SIgA of upper respiratory tract mucosa in model mice. Methods: The models of flora were established by intraperitoneal injection of penicillin to observe the effect of Panax notoginseng saponins on the regulation of SIgA in the respiratory mucosa of mice. The experiment was divided into 3 groups: blank (negative control) group, penicillin model group, Panax notoginseng saponin group. Results: In mice given intraperitoneal injection of penicillin for 3 days, streptococcus mutans, the flora density and flora diversity were significantly decreased, suggesting that the model was successfully replicated. Panax notoginseng saponin significantly increased SIgA of upper respiratory mucosa, which was reduced by penicillin-induced model mice. Conclusion: The penicillin-induced upper respiratory tract dysbacteriosis model can be used to study the regulation of mouse upper respiratory tract infection by Chinese medicine. One of the mechanisms of the prevention and treatment of upper respiratory tract infection by Panax notoginseng saponins is to promote the secretion of SIgA , Enhance the local immune function of the upper respiratory tract mucosa.