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用对流免疫电泳(CIE)检测痰中肺炎球菌多糖(PPS),和血清及尿中所检测的PPS以及痰的肺炎球菌培养结果对比,证实CIE对诊断肺炎球菌性肺炎很有价值。做CIE时痰液先经乙酰半胱氨酸液化,所用抗血清乃是包括所有已知83型的肺炎球菌多价抗血清,以及4～5个型别所组合的抗血清,如电泳洲出PPS,再进一步用单价的特异血消来鉴定型别。 41例患者分为4组。Ⅰ组:血培养阳性确诊为肺炎球菌性肺炎,Ⅱ组:符合肺炎球菌肺炎诊断标准,很可能为肺炎球菌性肺炎,痰固紫染色镜检以肺炎球菌形态的细菌占优势;Ⅲ组:不肯定为肺炎球菌性
Confirmation of the CIE in the diagnosis of pneumococcal pneumonia was confirmed by CIE in detecting pneumococcal polysaccharide (PPS) in sputum, in comparison with the PPS detected in serum and urine, and pneumococcal culture of sputum. When doing CIE sputum by acetylcysteine liquefaction, antisera but include all known type 83 pneumococcal polyvalent antiserum, as well as 4 to 5 types of combinations of antisera, such as electrophoresis out PPS, and then further monovalent specific blood consumption to identify type. 41 patients were divided into 4 groups. Group Ⅰ: positive blood culture diagnosed as pneumococcal pneumonia, group Ⅱ: in line with the diagnostic criteria of pneumococcal pneumonia, pneumococcal pneumonia is likely, sputum purple staining of bacteria in the form of pneumococcal dominant; Ⅲ group: not Certainly pneumococcal