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目的观察伞兵跳伞应激对唾液α-淀粉酶活性的影响,为使用唾液α-淀粉酶评定跳伞应激水平提供方法和生理依据。方法选取跳伞新兵50名(跳伞组),分别在登机前2h、登机前1h、着陆后0.5h以及着陆后2h采集唾液标本,检测唾液中α-淀粉酶活性和皮质醇浓度;另外选取不参加跳伞的伞兵20名(对照组),完成对照测量。结果从登机前2h至着陆后0.5h,跳伞组唾液皮质醇水平明显升高,每个时间点唾液皮质醇浓度均高于对照组;着陆后2h皮质醇水平虽仍高于对照组,但已经明显低于登机前2h时的水平。与唾液皮质醇浓度变化趋势相同,跳伞组α-淀粉酶活性在着陆后0.5h检测值最大,着陆后2h活性水平明显降低,不同时间点其活性水平也都高于对照组。跳伞前后唾液α-淀粉酶活性变化与皮质醇浓度变化呈明显正相关。结论跳伞应激对唾液α-淀粉酶活性有明显影响,唾液α-淀粉酶活性可以作为评估跳伞应激水平的重要生化指标。
Objective To observe the effect of paragliding parachute stress on salivary α-amylase activity, and to provide methods and physiological basis for saliva α-amylase evaluation of parachute stress. Methods Fifty parachuting recruits (parachuting group) were selected, saliva samples were collected 2h before boarding, 1h before boarding, 0.5h after landing and 2h after the landing, respectively. The salivary α-amylase activity and cortisol concentration were also measured. Twenty paratroopers who did not participate in the parachute (control group) completed the control survey. Results The saliva cortisol levels in the parachuting group increased significantly from 2 hours before boarding to 0.5 hours after landing, and the concentrations of salivary cortisol in the parachuting group were higher than those in the control group at each time point. Although the level of cortisol was still higher than that of the control group at 2 hours after landing, Already significantly lower than at 2h before boarding. The same as salivary cortisol concentration, the α-amylase activity in the parachute group was the highest at 0.5 h after landing and significantly lower at 2 h after landing, and its activity was also higher than that of the control group at different time points. Changes in saliva α-amylase activity before and after parachuting and cortisol concentration was positively correlated. Conclusion Parachute stress has a significant effect on the salivary α-amylase activity, salivary α-amylase activity can be used as an important biochemical indicators to assess the level of parachute stress.