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芦笋即百刁柏(Asparagus officinalisL.)是雌雄异株多年生宿根草本植物,雄株比雌株产量高25—30％。有性繁殖群体的性比为1∶1,雌、雄株在种子上缺乏明显的遗传标志,难于辨识,从而限制了产量的提高。为此,培育全雄植株,已成为芦笋研究的一个重要课题,用组培的方法为实现芦笋全雄化栽培提供了可能,但生产大量具备自营生活力的小植株的实用化组培技术至今还未解决。为此我们进行了 AP 对芦笋腋芽分离培养作用的研究,并取得了显著效果。此方面研究国内外尚未见报道。材料与方法
Asparagus (Asparagus officinalis L.) is a dioecious perennial herb with a male plant yield 25-30% higher than that of the female plant. Sexual reproduction population sex ratio of 1: 1, the female and male plants lack the obvious genetic markers in the seed, difficult to identify, thus limiting the yield increase. Therefore, to cultivate all male plants has become an important issue in the research of asparagus, and it is possible to realize the all-male cultivation of asparagus with the method of tissue culture. However, the practical technique of tissue culture for producing a large number of plantlets with self-supporting vitality So far still unresolved. To this end, we carried out AP on the axillary buds of Asparagus isolated culture research, and achieved remarkable results. This area has not been reported at home and abroad. Materials and Methods