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目的探讨重组人促红细胞生成素(recombinant human erythropietin,r-HuEPO)对癫痫大鼠海马齿状回的影响。方法 140只大鼠按随机数字表法分为正常对照组20只,余120只制作氯化锂-匹罗卡品颞叶癫痫模型,符合条件者采用随机数字表将其分为癫痫治疗组和癫痫组;各组均经腹腔注射BrdU,治疗组同时予以r-HuEPO处理;以FJ-B(Fluoro-Jade B)免疫荧光观察神经元变性情况,BrdU免疫组化观察细胞增殖情况,Timm’s染色观察齿状回苔藓纤维发芽情况。结果海马齿状回神经元变性在癫痫组(32.32±5.09)较治疗组(11.12±0.75)和对照组(6.50±1.08)明显(P<0.01),而治疗组和对照组相比无显著差异;第9天细胞增殖在癫痫组(92.8±2.97)较治疗组(16.95±0.54)和对照组(12.80±2.13)明显(P<0.01),治疗组和对照组相比无显著差异;第28天细胞增殖在癫痫组(146.45±5.91)较治疗组(46.27±2.93)和对照组(38.52±3.10)明显(P<0.01),治疗组和对照组相比无显著差异;Timm’s染色结果表明在第14天,癫痫组(0.45±0.19)苔藓纤维发芽比对照组(0.10±0.11)明显(P<0.05),第28天癫痫组(3.60±0.16)比治疗组(0.55±0.10)和对照组(0.30±0.11)更加明显(P<0.01),治疗组与对照组比差异不明显(P>0.05)。结论癫痫能诱发大鼠海马齿状回神经元变性、细胞增殖和苔藓纤维发芽;r-HuEPO处理能降癫痫大鼠海马齿状回神经元变性和细胞增殖,并能抑制苔藓纤维发芽。
Objective To investigate the effect of recombinant human erythropietin (r-HuEPO) on hippocampal dentate gyrus in epileptic rats. Methods 140 rats were divided into normal control group (n = 20) according to random number table method, and the other 120 rats were made lithium-pilocarpine-induced temporal lobe epilepsy model. Those who were eligible were divided into epilepsy treatment group Epilepsy group. BrdU was injected intraperitoneally in each group. The treatment group was treated with r-HuEPO at the same time. FJ-B (Fluoro-Jade B) immunofluorescence was used to observe the degeneration of neurons. BrdU immunohistochemistry was used to observe the proliferation of cells. Germination of the dentate gyrus moss fiber. Results The degeneration of dentate gyrus neurons in hippocampus was significantly (P <0.01) in the epilepsy group (32.32 ± 5.09) compared with the control group (11.12 ± 0.75) and the control group (6.50 ± 1.08), while there was no significant difference between the treatment group and the control group ; On the 9th day, the cell proliferation was significantly higher in the epilepsy group (92.8 ± 2.97) than in the treatment group (16.95 ± 0.54) and in the control group (12.80 ± 2.13) (P <0.01), and there was no significant difference between the treatment group and the control group Compared with the control group (46.27 ± 2.93) and the control group (38.52 ± 3.10), the proliferation of day cells in the epilepsy group (146.45 ± 5.91) was significantly (P <0.01). There was no significant difference between the treatment group and the control group On the 14th day, the mossy fiber germination in epilepsy group (0.45 ± 0.19) was significantly higher than that in the control group (0.10 ± 0.11) and epilepsy group (3.60 ± 0.16) on the 28th day (0.55 ± 0.10) and the control group (0.30 ± 0.11) (P <0.01). The difference between the treatment group and the control group was not significant (P> 0.05). Conclusion Epilepsy can induce neuron degeneration, cell proliferation and mossy fiber germination in rat hippocampal dentate gyrus neurons. R-HuEPO treatment can degenerate and proliferate the dentate gyrus neurons in the hippocampus of rats with epilepsy and inhibit the germination of mossy fibers.