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目的:研究EDTA、CaCl2、红酵母酸和肠菌素对Botrytis cinerea多聚半乳糖醛酸酶(PG)和漆酶(LC)的抑制作用。方法:分离得到2株具有致病性、能抵抗杀菌剂以及分泌PG和LC的灰葡萄孢菌。观察四种抑制剂对B.Cinerea感染苹果的防治效果。将菌种活化后分别给予四种抑制剂(EDTA、CaCl2、红酵母酸和肠菌素)处理7d后,测定不同组别菌体干重和总蛋白含量。利用酶动力学的方法比较四种抑制剂EDTA、CaCl2、红酵母酸和肠菌素对两株灰葡萄孢菌中PG和LC活性的影响。结果:EDTA和红酵母酸能显著提高健康苹果对B.Cinerea感染的抵抗能力,而氯化钙和肠菌素则对已感染了B.Cinerea的苹果感染面积的扩大有较好的控制作用。肠菌素对两菌株LC酶活性的抑制作用最强、抑制率达70-80%,氯化钙对PG活性的抑制作用最强、抑制率达45%。结论:EDTA和红酵母酸可预防B.Cinerea的感染,肠菌素和氯化钙对B.Cinerea的感染有治疗作用。其防治作用可能与抑制B.Cinerea中PG和LC的活性有关。
OBJECTIVE: To study the inhibitory effects of EDTA, CaCl2, Rhodotorula glutamic acid and enterocet on Botrytis cinerea polygalacturonase (PG) and laccase (LC). Methods: Two strains of Botrytis cinerea that were pathogenic, resistant to bactericide and secreted PG and LC were isolated. To observe the four inhibitors of B.Cinerea infection in apple control effect. After activation of the strain, four kinds of inhibitors (EDTA, CaCl2, erythromycin acid and enterocin) were given for 7 days respectively to determine the dry weight and the total protein content of the different groups of bacteria. The effects of four inhibitors EDTA, CaCl2, Rhodotorula glutamic acid and enterocetin on the activities of PG and LC in two strains of Botrytis cinerea were compared by enzyme kinetics. Results: EDTA and Rhodotorula acid could significantly improve the resistance of healthy apple to B.Cinerea infection, while calcium chloride and enterocin had a good control effect on the expansion of apple infected area infected with B.Cinerea. Entericillin had the strongest inhibitory effect on the activity of LC in two strains, with the inhibitory rate reaching 70-80%. Calcium chloride had the strongest inhibitory effect on the activity of PG with the inhibition rate of 45%. CONCLUSION: EDTA and Rhodotorula are effective in preventing the infection of B. Cinerea, and enterocin and calcium chloride have a therapeutic effect on B. Cinerea infection. Its prevention and treatment may be related to inhibiting the activity of PG and LC in B.Cinerea.