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目的建立H P L C法测定速克感冒胶囊中游离水杨酸的含量。方法色谱柱:Diamonsil C18色谱柱(4.6mm×150mm,5μm),以甲醇-0.1%二乙胺水溶液-冰醋酸(40:60:4)为流动相,检测波长为235nm,进样量:20μl,柱温:25℃,流速:1ml·min-1。结果水杨酸在4~80μg·ml-1范围内与峰面积线性关系良好(r=0.9995),平均回收率99.3%(RSD=1.3%,n=9),水杨酸检测限为1ng。结论本方法准确可靠,能为速克感冒胶囊质量的控制提供依据。
OBJECTIVE To establish a HPLC method for the determination of free salicylic acid in Shukong capsule. Methods The mobile phase was Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) with methanol-0.1% diethylamine in glacial acetic acid (40:60:4) as the mobile phase. The detection wavelength was 235 nm. The injection volume was 20 μl , Column temperature: 25 ° C, flow rate: 1 ml · min-1. Results The salicylic acid showed a good linearity (r = 0.9995) with an average recovery of 99.3% (RSD = 1.3%, n = 9) in the range of 4-80 μg · ml-1 and the detection limit of salicylic acid was 1 ng. Conclusion This method is accurate and reliable, which can provide the basis for the quality control of Sankker.