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应用多聚酶链反应和单链构象多态性分析法（ＰＣＲ－ＳＳＣＰ）检测１９例冰冻胃癌组织Ｐ５３基因第７外显子ＤＮＡ片段扩增及突变情况，同时应用免疫组化法（ＩＨＣ）检测相应胃癌石蜡包埋组织中Ｐ５３蛋白的表达。结果发现４例（２１．１％）胃癌组织Ｐ５３基因第７外显子发生突变。而Ｐ５３蛋白表达率达４７．４％（９／１９）。Ｐ５３基因突变及Ｐ５３蛋白表达与肿瘤发生部位、组织学类型、淋巴结转移及肿瘤分期无关（Ｐ＞０．０５）。提示在胃癌发生和演变过程中，Ｐ５３基因突变是重要事件，其中第７外显子是突变热区之一。
The amplification and mutation of exon 7 of P53 gene in 19 cases of frozen gastric cancer tissues were detected by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP). The corresponding immunohistochemical method (IHC) was used to detect the corresponding mutations. Expression of P53 protein in paraffin - embedded tissues of gastric cancer . The results revealed mutations in exon 7 of the P53 gene in gastric cancer in 4 patients (21.1%). The expression rate of P53 protein was 47.4% (9/19). The mutation of P53 gene and the expression of P53 protein were not associated with tumor site, histological type, lymph node metastasis and tumor stage (P>0.05). The p53 gene mutation is an important event during the development and progression of gastric cancer. The exon 7 is one of the hot regions of mutation.