论文部分内容阅读
为了进一步研究水稻晚粳品种农垦 5 8转变为光敏核不育水稻农垦 5 8S的突变位点 ,并精细定位光敏不育基因pms3,我们利用农垦 5 8S/15 14杂交组合的 F1 进行花药培养构建了一个 DH群体 ,验证了在该群体中光敏不育受 pms1、pms3两对基因的控制 ,并根据分子标记分析选择第 12染色体是 15 14基因型、第 7染色体农垦 5 8S基因型的 DH系 DH80与农垦 5 8S杂交构建一个 pms3光敏不育单基因分离的群体。根据 F3家系的育性分离情况推测出 F2 的基因型 ,结合分子标记分析进一步验证了 pms3在第 12染色体上的位置 ,并将其定位在 RFL P标记 M36和 RZ2 6 1之间 ,与两标记的遗传距离分别为 1.5 c M和 3.0 5 c M,为启动 pms3区域的染色体步查打下了基础。同时还比较分析了 RFL P标记在 DH群体及F2 群体中的分离情况 ,发现第 12染色体上 pms3区域在 F2 群体中正常分离的多个标记在 DH群体中发生了严重的偏分离 ,另外还发现 DH群体第 3、 7、 11染色体上的标记也发生了偏分离
In order to further study the transformation of late japonica rice cultivar Nongken58 to the mutant site of S585 of NENken58 and the fine mapping of the photo-sensitive sterile gene pms3, we constructed the anther culture with F1 of Nongken 58S / 15 14 In a DH population, it was verified that the photo-sterility in this population was controlled by two pairs of pms1 and pms3 genes. According to the molecular marker analysis, the 12th chromosome was selected as 1514 genotype. The DH strain of genotype 7 Nongken588 DH80 and Nongken5 8S were crossed to construct a population of pms3 photogenerated sterile single gene. The genotype of F2 was deduced based on the fertility segregation of F3 families. Combined with molecular marker analysis, the position of pms3 on chromosome 12 was further confirmed and located between RFL P markers M36 and RZ2 6 1, The genetic distances of 1.5 c M and 3.0 5 c M, respectively, laid the foundation for the initiation of chromosome sequencing of the pms3 region. In addition, the segregation of RFL P markers in DH population and F2 population was comparatively analyzed. It was found that a number of markers normally segregating in the F2 population of pms3 region on chromosome 12 were severely segregated in the DH population. In addition, The segregation of markers on chromosomes 3, 7 and 11 of DH population also occurred