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We describe a new species of the genus Megophrys from Guizhou Province,China.Molecular phylogenetic analyses based on mitochondrial DNA and nuclear DNA sequences all strongly supported the new species as an independent clade nested into the Megophrys clade and sister to M.minor.On morphology,the new species is distinguished from its congeners by a combination of the following characters:(1) small body size with SVL ＜ 392 mm in male and SVL ＜ 40.4 mm in female;(2) vomerine teeth absent;(3) tonguenot notched behind;(4) a small h-like tubercle at the edge of each upper eyelid;(5) tympanum distinctly visible,rounded;(6) two or three metacarpal tubercles in hand;(7) relative finger lengths:Ⅰ ＜ Ⅱ ＜ Ⅴ ＜ Ⅲ;(8) toes without webbing;(9) heels overlapped when thighs are positioned at right angles to the body;(10) tibiotarsal articulation reaching tympanum to eye when leg stretched forward;(11) an intal single subgular vocal sac in male;(12) in breeding male,the nuptial pads with black nuptial spines on the dorsal bases of the first and second fingers.