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目的了解慢性应激对大鼠结肠杯状细胞粘液及粘蛋白含量的影响。方法健康成年Wistar大鼠24只,随机分为实验组和对照组,对照组大鼠自由活动,实验组每天置于束缚笼内限制活动2h,持续14d后处死,粘液组化AB/PAS法观察结肠黏膜含粘液杯状细胞的数目、免疫组化方法测定粘蛋白muc2的蛋白表达情况。结果重复应激引起大鼠结肠含粘液杯状细胞数目减少(33.00±6.31vs47.42±8.06)(t=4.878,P<0.01),杯状细胞内粘蛋白muc2蛋白表达减少(0.22±0.03vs0.26±0.02)(t=4.537,P<0.01)。结论慢性应激影响肠黏膜粘液分泌细胞的功能,损伤肠粘液保护屏障。
Objective To investigate the effects of chronic stress on the content of mucus and mucin in colonic goblet cells in rats. Methods Twenty-four healthy adult Wistar rats were randomly divided into experimental group and control group. The rats in the control group were free-living. The rats in the experimental group were restrained for 2 hours in the cage and were sacrificed 14 days later. The mucosa were observed by AB / PAS Colonic mucosa with mucinous goblet cells in the number of immunohistochemical method mucin muc2 protein expression. Results The number of mucus mucin in goblet cells decreased (33.00 ± 6.31 vs 47.42 ± 8.06) (t = 4.878, P <0.01), and the expression of muc2 in goblet cells decreased (0.22 ± 0.03 vs0 .26 ± 0.02) (t = 4.537, P <0.01). Conclusion Chronic stress affects the function of mucus secreting cells in intestinal mucosa and damages the intestinal mucosal protective barrier.