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目的 :研究参与炎症的环氧化酶 -2(Cox 2 )和凋亡相关蛋白caspase 3在大鼠肺鳞癌发生发展各阶段的蛋白表达状况并探讨炎症、凋亡和肺癌发生发展的关系。方法 :Wistar大鼠 80只 ,左肺叶支气管灌注含三甲基胆蒽(MCA)和二乙基亚硝胺 (DEN )的碘油溶液 ,分批处死获取肺鳞癌形成过程各阶段标本。免疫组化检测各阶段病变组织Cox 2、caspase 3 的表达并计算免疫组化评分 (IHS)。结果 :79 0 0 %( 4 9/ 62 )的动物标本有多个阶段病变共存 ,共获取支气管膜上皮增生 14例 ,鳞状化生 2 5例 ,不典型增生 3 3例 ,原位癌 12例 ,侵袭癌 5 4例 ,转移癌 17例。正常支气管黏膜上皮偶有Cox 2弱阳性表达 ,随着癌变进展而逐步上调。不典型增生、原位癌及转移癌阶段Cox 2的IHS增高有统计学意义 ,P <0 0 1、P <0 0 5、P <0 0 1。 10例对照组大鼠支气管黏膜上皮细胞中 8例 ( 8/ 10 )有caspase 3的阳性表达 ,随着支气管黏膜上皮的癌变进程表达下降。不典型增生、转移癌阶段caspase 3的HIS降低有统计学意义 ,P <0 0 5、P <0 0 1。在 165例大鼠肺组织病变中 ,Cox 2与caspase 3呈显著负相关 ,χ2 =2 1 675 ,spearman相关系数为 -0 2 46,P =0 0 0 1。结论 :Cox 2、caspase 3均与肺鳞癌的发生和发展过程相关 ,可能是联系炎症、凋
AIM: To investigate the expression of Cox 2 and apoptosis-related protein caspase 3 in various stages of lung squamous cell carcinogenesis and to explore the relationship between inflammation, apoptosis and lung cancer. Methods: 80 Wistar rats were infused with lipiodol solution containing trimethylchloroacene (MCA) and diethylnitrosamine (DEN) into the bronchus of the left lobe and were sacrificed in batches for different stages of squamous cell carcinomas. Immunohistochemistry was used to detect the expression of Cox 2, caspase 3 and the immunohistochemical score (IHS). Results: 79 0 0% (49/62) of the animal specimens had multiple stages of coexistence of lesions, and 14 cases of bronchial epithelial hyperplasia, 25 cases of squamous metaplasia, 33 cases of dysplasia and 12 cases of carcinoma in situ Cases, invasion of 54 cases of cancer, 17 cases of metastatic cancer. Normal bronchial mucosa epithelial occasional Cox 2 weakly positive expression, with the progression of cancer gradually increased. There was significant difference in IHS of Cox 2 between atypical hyperplasia, carcinoma in situ and metastasis stage (P <0.01, P <0.05, P <0.01). The positive expression of caspase 3 was found in 8 of 10 (10/8) bronchial epithelial cells of 10 control rats, with the decrease of bronchial epithelium carcinogenesis. HIS of atypical hyperplasia and metastasis stage of caspase 3 had statistical significance, P <0.05, P <0.01. There was a significant negative correlation between Cox 2 and caspase 3 in lung tissue of 165 rats (χ2 = 21 675, spearman correlation coefficient -0 2 46, P = 0 0 01). Conclusion: Both Cox 2 and caspase 3 are associated with the occurrence and development of squamous cell carcinomas, which may be related to inflammation and apoptosis