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目的研究广州妇女宫颈感染HPV主要基因型及病毒种系发生。方法采集医院宫颈专科就诊妇女宫颈脱落细胞,PCR检测HPV感染及其基因型;通过特异性扩增技术获取人乳头瘤病毒早基因E6/E7的编码区序列,克隆测序并对序列进行分析;通过GenBank检索获取相应序列,用DNAStar进行比对和种系发生分析。结果 1 542例妇女的宫颈脱落细胞标本检出HPV阳性390例,主要高危型病毒为HPV52、HPV16和HPV58。对HPV16采用E6E7基因序列进行进化分析,与使用HPV全基因组序列、L1ORF序列的结果基本一致。E6E7基因序列分析,显示本地区HPV52在种系发生上多聚集于B亚群,HPV16与欧洲株接近而不同于亚洲-美洲株及非洲株,HPV58主要分布于A亚群。结论广州地区妇女宫颈感染的HPV基因型主要为52、16和58型,可采用E6E7基因序列对HPV进行种系发生研究,种系发生具有地方性特征。
Objective To study the main HPV genotypes and viral germline infection in Guangzhou women. Methods Cervical exfoliated cells were collected from women with cervical specialties in hospital. The HPV infection and genotypes were detected by PCR. The coding region of early gene E6 / E7 of human papillomavirus was obtained by specific amplification and cloned and sequenced. GenBank access to obtain the corresponding sequence, DNAStar alignment and phylogenetic analysis. Results A total of 390 HPV positive cases were detected in 1 542 women with cervical exfoliated cells. The major high-risk viruses were HPV52, HPV16 and HPV58. The evolutionary analysis of HPV16 using the E6E7 gene sequence was basically consistent with the results of using the HPV genome and L1ORF sequences. E6E7 gene sequence analysis showed that HPV52 mainly clustered in subgroup B in germline development. HPV16 was similar to European strain but different from that of Asian-American strain and African strain. HPV58 mainly distributed in subgroup A. Conclusion The HPV genotypes of cervical infections in women in Guangzhou are mainly 52, 16 and 58 types. E6E7 gene sequences can be used to study the germline development of HPV, and the germ line is endemic.