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通过PCR、克隆文库方法分析了农业有机废物和城市垃圾堆肥高温期间细菌和真菌种群的多样性。提取堆肥高温期的DNA,PCR扩增,构建各高温期的16S rDNA和18S rDNA克隆文库,结果表明:农业有机废物和城市生活垃圾16S rDNA克隆文库中分别共有18个、21个OTUs,分别属于细菌域的14个、15个不同属,其18S rDNA克隆文库中分别共有8个、9个OTUs,分别属于细菌域的8个、9个不同属,推断农业有机废物堆体的优势菌为Bacillus megaterium、Rhizobium sp.、Phanerochaete chrysosporium、Penicillium sp.同属或同种的菌株;城市生活垃圾堆体的优势菌为Bacillus megaterium、Azospirillum sp.、Phanerochaete chrysosporium同种或同属的菌株。
The diversity of bacterial and fungal populations during the high temperature of agricultural organic waste and municipal waste composting was analyzed by PCR and cloning libraries. The 16S rDNA and 18S rDNA clone libraries of each 16S rDNA and 18S rDNA clone library were extracted from the composting high temperature stage. The results showed that there were 18 and 21 OTUs in the 16S rDNA clone library of agricultural organic waste and municipal solid waste respectively, belonging to There were 14 and 15 different genera in the bacterial community. There were 8 and 9 OTUs in the 18S rDNA clone library, which belonged to 8 and 9 different genus respectively. It was concluded that the dominant bacteria in the organic wastes were Bacillus Megaterium, Rhizobium sp., Phanerochaete chrysosporium and Penicillium sp. The dominant bacteria of municipal solid waste are Bacillus megaterium, Azospirillum sp., and Phanerochaete chrysosporium.