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1868年Langerhans所发现的细胞,很可能因为应用通常的组织学方法难以见到而被忽视.Langerhans发现它们可被氯化金着色,遂认为其系神经细胞.1875年Ranvier指出这些细胞系淋巴细胞来源,其后多年来曾被当作是表皮细胞、人为现象、黑素细胞以及诸如许旺氏细胞的神经成分.1961年Birbeck等描述了一种特殊细胞器,此种细胞器或颗粒现在可用电镜观察到.自从Langerhans细胞(LCs)能被确凿地识别后,即开始研究其结构和功能.用光镜显示LCs的方法已有所改进,包括金、锇、钴、镧、汞、镍和铬等金属皆可使之着色.它们可摄取诸如亚甲基蓝及亮甲苯蓝等体外活体染料以及诸如甲醛、戊二醛、乙烯-二胺等作为接触性抗原,可以组化方法予以识别.LCs对各种不同的儿茶酚胺类如多巴胺、正肾上腺素及L-DOPA有亲和力.将皮肤在体外接触这些物质后,再暴露于甲醛
Cells discovered by Langerhans in 1868 were most likely to be overlooked because they were difficult to see by conventional histological methods and Langerhans found that they were neuronal cells that were colored with gold chloride. Ranvier pointed out in 1875 that these cell lines were lymphocytes Sources, which have for centuries been considered epidermal cells, artifacts, melanocytes, and neural components such as Schwann cells, Birbeck et al. Described a particular organelle in 1961 that is now electron microscopically observable Since Langerhans cells (LCs) have been able to identify their structure and function, LCs have been improved by light microscopy, including gold, osmium, cobalt, lanthanum, mercury, nickel and chromium Metals can be colored.They can absorb in vitro living dyes such as methylene blue and bright toluene blue as well as contact antigens such as formaldehyde, glutaraldehyde, ethylene-diamine and the like, and can be identified by histochemical methods. Of catecholamines such as dopamine, norepinephrine and L-DOPA have affinity to the skin in vitro exposure to these substances, and then exposed to formaldehyde