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近年来,将不同生物的具有已知遗传信息的DNA片段导入受体生物细胞内,并使之表达的研究正在方兴未艾。尽管有些人对这类工作的结果提出质疑,引起争论,可这几年来,许多用植物组织培养系统做的遗传转化研究工作相继表明,外源DNA片段可以通过转化的手段导入植物细胞内,部分片段似还可被受体细胞DNA整合乃至表达。 在我国,自1977年底起开展协作,用小麦、水稻、棉花等作为受体亲本,配成各种亲缘不同的组合,进行外源DNA片段的导入研究,初步获得了一些结果。本文报道了利用外源海岛棉DNA注射导入陆地棉后,在其子代中产生变异的情况。
In recent years, the study of introducing and expressing DNA fragments of different organisms with known genetic information into recipient biological cells is in the ascendant. Although some people have questioned and controversially raised the result of such work, many researches conducted in the field of genetic transformation using plant tissue culture systems in recent years have shown that exogenous DNA fragments can be introduced into plant cells by transformation means. In part Fragments may also be receptor DNA integration and even cell expression. In our country, since the end of 1977, there have been some collaborations, using wheat, rice, cotton and so on as the recipient parents, dubbing various combinations of kinship and carrying out the introduction of exogenous DNA fragments. This article reports the use of exogenous island cotton DNA inoculation into G. hirsutum, resulting in variation in their offspring.