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Fe3O4 magnetic nanoparticles with diameters varying from 10 to 426 nm were synthesized and characterized. Heating effects of Fe3O4 magnetic nanoparticles under radiofrequency capacitive field (RCF) with frequency of 27.12 MHz and power of 60-150 W were investigated. When the power of RCF is lower than 90 W, temperatures of Fe3O4 magnetic nanoparticles (75-150 mg/mL) can be raised and maximal temperatures are all lower than 50 癈. When the power of RCF is 90 150 W, temperatures of Fe3O4 magnetic nanoparticles can be quickly raised and are all obviously higher than those of normal saline and distilled water under the same conditions. Temperature of Fe3O4 magnetic nanoparticles can even reach 70.2 ℃ under 150 W RCF. Heating effects of Fe3O4 magnetic nanoparticles are related to RCF power, particle size and particle concentration.