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目的探讨地黄管食通口服液对食管癌放疗大鼠模型的影响及作用机制。方法 Wistar大鼠20只为空白组,110只皮下注射甲基戊基亚硝铵5mg/kg建立食管癌模型。除食管癌组20只外,其余分为5组,均应用60Co食管局部放疗。单纯放疗组(单放组)18只,其余分成六味组(18只,浓度0.45g/ml六味地黄丸混悬液放疗后灌胃10ml/kg),地黄管食通高、中、低剂量组(各18只,浓度分别为1.5g/ml、1.0g/ml、0.5g/ml地黄管食通口服液放疗后灌胃10ml/kg)。空白组、食管癌组、单放组每天灌服蒸馏水10ml/kg,各组均灌胃5周。镜下观察各组大鼠食管端粒酶逆转录酶(hTERT)形态学及阳性率比较。结果镜下地黄管食通高、中、低剂量组及六味组胞核及细胞浆呈棕黄色着染,着色明显比食管癌组浅。与食管癌组比较,各药物组hTERT阳性率差异均有统计学意义(P<0.01);高剂量组与单放组比较差异有统计学意义(P<0.05);各药物组之间比较差异无统计学意义(P>0.05)。结论地黄管食通口服液可能通过下调hTERT活性表达从而防治食管癌放疗后复发。
Objective To investigate the effect and mechanism of Dihuang Guan Shi Tong oral liquid on esophageal cancer radiotherapy rat model. Methods Twenty Wistar rats were used as blank control group, 110 rats were injected subcutaneously with 5 mg / kg methyl amyl ammonium nitrite to establish esophageal cancer model. In addition to esophageal cancer group of 20, the remaining divided into 5 groups, are applied 60Co esophageal radiotherapy. 18 rats in the radiotherapy alone group (the control group) were divided into six groups (18 rats, the concentration of 0.45g / ml Liuweidihuangwan suspension after intragastric administration of 10ml / kg) Each of 18, the concentration of 1.5g / ml, 1.0g / ml, 0.5g / ml Dihuang Guan Shi Tong oral solution after oral administration of gavage 10ml / kg). The blank group, esophageal cancer group, single release group were given distilled water 10ml / kg every day, each group were fed for 5 weeks. The morphology and positive rate of esophageal telomerase reverse transcriptase (hTERT) in each group were observed under microscope. Results Microscope tube food-high, medium and low dose group and Liuwei group nucleus and cytoplasm brown stained, staining was significantly lighter than the esophageal cancer group. Compared with the esophageal cancer group, the positive rate of hTERT in each drug group was significantly different (P <0.01), the difference was statistically significant (P <0.05) between high-dose group and single-drug group No statistical significance (P> 0.05). Conclusion Dihuang Guan Shi Tong oral liquid may prevent recurrence of esophageal cancer after radiotherapy by down-regulating the expression of hTERT activity.